6. březen 2019 829 vyz

Thesis for Enrollment AY 2019/2020

Study Program Thesis Tutor
Experimental Surgery

Study of new therapeutic targets and anticancer drugs in experimental models and solid tumor patients

doc. RNDr. Pavel Souček, Ph.D.
 

Chromosomal damage and instability in solid cancers

MUDr. Pavel Vodička, CSc.
 

Repopulation of decellularized liver scaffold with cells: Development and optimalization of repopulation technique for engineering functional liver tissue

RNDr. Vladimíra Moulisová, Ph.D.
  Characterization of decellularized liver scaffold structure and cell – scaffold interactions as prerequisites for liver tissue engineering RNDr. Vladimíra Moulisová, Ph.D.
 

Characterising immune cells in colorectal tumors

doc. MUDr. Václav Liška, Ph.D.
 

Genetics of colorectal cancer progression

doc. MUDr. Václav Liška, Ph.D.
Medical Microbiology

Development and validation of novel molecular assays for rapid diagnosis of infectious diseases

Time around time of microbiological diagnostic methods seriously affects early administration of focused therapy. Within the project, novel technologies based on MALDI-TOF mass spectrometry and third generation nucleic acid sequencing (Pacific Biosciences) will be developed and validated. Those methods should provide a rapid response to an infection. MALDI-TOF mass spectrometric assays will be mainly focused on functional detection of bacterial toxins (e.g. Clostridium difficile toxins, Yersinia enterocolitica toxins) and mechanisms of antibiotic resistance (e.g. carbapenemase production, polymyxin resistance). Genomic analysis will use whole-genome sequencing of pathogens as well as cells of immune system to develope efficient tools for prediction a host-pathogen interaction and antiinfective therapy.   

doc. Ing. Jaroslav Hrabák, Ph.D.
 

 Microbial genomics for population studies of multiresistant Gram-negative bacteria

The activities of the Ph.D. project will be mainly focused on genomic analysis of mechanisms of resistance to antibiotics, especially to expanded-spectrum cephalosporins and carbapenems, in Gram-negative bacteria (Enterobacterales, Pseudomonas spp.). Resistant isolates will be collected in Czech hospitals within EURGene Net project CCRE – isolates resistant to carbapenems and colistin. In the collection, molecular epidemiological factors affecting dissemination of resistance among nosocomial pathogens will be determined. Methodological approaches will also include analysis of genetic carriers of resistance determinants and whole-genome sequencing (all isolates) using Illumina and Pacific Biosciences platforms.

 Ibrahim Bitar, Ph.D.
 

Genomic analysis of pathogenic Escherichia coli strains resistant to antimicrobials using new generation sequencing approach and bioinformatics

Increasing incidence of bacteria resistant to antimicrobial agents and their global spread is one of the most important threat to current medicine, causing serious difficulties in the therapy of infectious diseases.  Growing resistance has been documented also in pathogenic strains of Escherichia coli that cause various infections in humans and animals. Genomics and whole genome sequencing (WGS) have the capacity to greatly enhance knowledge in microbiology and they have become an important approach in our understanding of traits and specific features of antibiotic resistant bacteria. However, current WGS technologies also pose new challenges for sequence analysis due to the enormous complexity, plasticity and diversity of bacterial genomes. The aim of the PhD study will be to work with data from NGS systems (Illumina, Pacific Biosciences, Oxford Nanopore), to design and apply in silico methods for bioinformatics processing of WGS data.

 

doc. RND. Monika Dolejská, Ph.D.
 

Gram-negative bacteria resistant to clinically important antibiotics in the environment and One Health concept

Transmission of bacteria resistant to clinically important antimicrobial agents is an important issue with serious consequences for human and animal health. The environment is considered as a source, reservoir and vector of these bacteria. Dissemination of resistant bacteria from hospitals and the community to aquatic environment is mainly via wastewater treatment plants from which treated waste water goes to rivers. Waste waters also represent an important source of infectious agents for wildlife, posing a significant impact on the environment. High prevalence of antibiotic resistant bacteria is also documented in other terrestrial species of wild animals that live closely to humans and in the environment contaminated by anthropogenic activities and feed on landfills, fields fertilized by manure and around wastewater treatment plants. The occurrence of gramnegative bacteria (mainly Escherichia coli, Klebsiella pneumoniae, Pseudomonas spp.) resistant to clinically important antimicrobials for human and veterinary medicine in waste waters, rivers and populations of wild animals will be monitored. Bacteria will be characterized using both basic and advanced microbiological techniques. Genetic basis pf resistance will be studied also by whole-genome sequencing using Illumina, PacBio and Oxford Nanopore techniques and the role of horizontal gene transfer in dissemination of antibiotic resistance. The aim of the thesis is to study the sources and transmission pathways of resistant bacteria using One Health concept that includes animals, humans and environment.

 

 

 

 

doc. RNDr. Monika Dolejská, Ph.D.

Rychlé odkazy

Výběrová řízení
Ukončení VŘ na pozici ERA Chair v projektu Chaperon

V Plzni dne 22.10.2019 čj.: UKLFP/249208/2019 - 7     ROZHODNUTÍ DĚKANA O VÝSLEDKU VÝBĚROVÉHO [...]

29. říjen 2019

Výběrová řízení
Ukončené výběrové řízení - Stomatologie

V Plzni dne 29.10.2019 Č. j.: UKLFP/ 269903/2019-5   ROZHODNUTÍ DĚKANA O VÝSLEDKU VÝBĚROVÉHO [...]

29. říjen 2019

Výběrová řízení
VŘ Odborný asistent I. interní klinika

Děkan Lékařské fakulty v Plzni, Univerzity Karlovy vypisuje výběrové řízení na obsazení [...]

29. říjen 2019 1

Důležité odkazy

UNIVERZITA KARLOVA

Oficiální stránky Univerzity Karlovy

WHOIS

Informační systém UK

STUDIJNÍ INFORMAČNÍ SYSTÉM

Studijní informační systém